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KMID : 1094020190360050259
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Journal of Veterinary Clinics
2019 Volume.36 No. 5 p.259 ~ p.265
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Effects of Alpha-G Rutin Supplementation in Sperm Freezing Extender on Dog Sperm Cryopreservation
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Park Sang-Hyoun
Jeon Yu-Byeol
Talha Nabeel Abdelbagi Hamad
Yu Il-Jeoung
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Abstract
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This study was designed to investigate the effects of alpha-glucosyl rutin (G-rutin) and its comparative effects with other antioxidants (glutathione: GSH, catalase: CATA and beta-mercaptoethanol : βME) on dog sperm freezing. In the first experiment (E1), the spermatozoa were diluted in freezing extender supplemented with 0 (control), 0.001, 0.01, or 0.1% G-rutin and frozen using liquid nitrogen (LN2). The progressive motility, reactive oxygen species (ROS) level and apoptosis of spermatozoa were assessed after sperm thawing at 37oC for 25 sec. In the second experiment (E2), 0.1% G-rutin group was compared with 10 mM β-ME, 5 μM GSH and 50 μM CATA groups by assaying progressive motility, viability and gene expression of Bcl-2 and SMCP after sperm freezing and thawing. In E1, 0.1% G-rutin group showed higher (P < 0.05) post-thaw progressive motility and lower (P < 0.05) ROS levels. In E2, the expressions of SMCP in G-rutin group were higher (P < 0.05) than in CATA group while Bcl-2 expression of Grutin group was higher (P < 0.05) than β-ME and CATA groups. However, there were no significant differences in progressive motility and viability. Therefore, we suggest that G-rutin can be used as a potentially antioxidative supplement in dog sperm freezing extender on the basis of gene expression related to motility and apoptosis as well as ROS level.
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KEYWORD
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antioxidant, dog, freezing extender, gene expression, G-rutin, reactive oxygen species, spermatozoa
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